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生物通
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全长 16S rRNA 测序:肥胖儿童代谢相关脂肪性肝病的肠道微生物组新探索
这些分类群是根据 16S rRNA 测序中相对丰度较高而选择的。结果显示,PCR 检测的相对数量与 FL16S 测序获得的相对丰度相关性良好,证明了分类学注释的准确性。同时,利用 ZymoBIOMICS Microbial Community DNA 标准对两种测序方法的准确性进行评估,发现两种方法都能较为 ...
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