PCR involves the enzymatic amplification of nucleic acid templates, and can be divided into four major steps, listed below. 1 Denaturation: Double-stranded DNA separates into single strands. Annealing ...

PCR amplifies DNA in a three-step process: denaturation, which melts double-stranded DNA into single strands; annealing, which lets small pieces of primer DNA bind to either side of the region of ...

PCR is very precise and can be used to amplify ... The mixture is then cooled so that the primers anneal, or bind, to the DNA template. At this point, the DNA polymerase begins to synthesize ...

By contrast, thermal cyclers use electric heating and cooling components to drive the PCR reaction through each of its three phases: denaturation, annealing and extension. During denaturation ...