For a 30-year-old experimental method, gel electrophoresis remains popular. Originally devised as a method to extract nucleic acids from solution, it has evolved into a method for the analysis of ...
Prepare a polyacrylamide minigel of the desired concentration containing 8 M urea (for example, Bio-Rad Mini ... Add 6 μl of gel-loading buffer and separate the DNA by electrophoresis through ...