这项研究颠覆了“PAM 越宽松越好”的传统认知，揭示特异性与效率的精准平衡才是关键。 CRISPR-Cas9 被誉为“基因魔剪”，但其编辑效率在不同场景 ...

本文聚焦于 CRISPR-Cas9 在微生物细胞工厂的应用。介绍了其作用机制和应用，探讨如何优化该技术，如降低脱靶效应、提高靶向效率，为推动此技术在微生物领域的发展提供全面视角，助力相关研发。 CRISPR-Cas9 技术概述 代谢工程改造的细菌菌株在众多生物基成分 ...

本文聚焦 Gasdermin E（GSDME）在癌症治疗中的作用。GSDME 参与细胞焦亡（pyroptosis），其表达异常与多种癌症相关。CRISPR-Cas9 技术可精准调控 GSDME。文章探讨其作为靶点和生物标志物的潜力、挑战及未来方向，为癌症治疗研究提供重要参考。 Gasdermin E（GSDME）与癌症 ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

Cas9, the first Cas protein repurposed for cutting DNA, introduces double-strand breaks that recruit DNA repair machinery. Despite its successes, the CRISPR-Cas9 system has several limitations, ...

CRISPR-Cas9, a gene editing technology known for its user-friendliness, can knock genes in or out. Knocking out a gene involves inserting CRISPR-Cas9 into a cell using a guide RNA that targets the ...

When the CRISPR Cas9 protein is added to a cell along with a piece of guide RNA, the Cas9 protein hooks up with the guide RNA and then moves along the strands of DNA until it finds and binds to a ...

Their results are published in Nature. "Our study is a first step in dramatically expanding our repertoire of effective and safe CRISPR-Cas9 enzymes. In our manuscript we demonstrate the utility ...

Collaborating with CRISPR-Cas9 co-inventor Jennifer Doudna and Jill Banfield at UC Berkeley, Steven Jacobsen, a distinguished professor of molecular, cell and developmental biology at UCLA ...

"Our study is a first step in dramatically expanding our repertoire of effective and safe CRISPR-Cas9 enzymes. In our manuscript, we demonstrate the utility of these PAMmla-predicted enzymes to ...