大家都知道，CRISPR/Cas9基因编辑技术在高效获得基因敲除突变（knockout）方面是非常有效的，但是想要通过同源重组修复途径 ...

实现更特异、更高效的基因敲入（knockin）！ CRISPR/Cas9和其它基因编辑工具已经被成功应用于获得基因敲除突变（knockout），但众多事实证明在基因敲入上的应用有很大的难度。基因敲入所面临的主要难题是修复模板的制备和如何与Cas9-sgRNA核糖核蛋白复合物共导入 ...

Zhu, Z., Verma, N., González, F., Shi, Z. D., & Huangfu, D. (2015). A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells. Stem Cell ...

Figure 1A. Schematic of the intron targeting-mediated strategy for generating hey2zCKOIS zebrafish by using the CRISPR/Cas9 system. Figure 1B. Schematic of the strategy for endothelial cell (EC ...

Feb. 13, 2025 — A new study unravels the relationship between mitofusins (mitochondrial proteins) and autophagy. Using gene editing with CRISPR-Cas9, the team has been able to study these ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

FinGEEC provides cell line generation and consultation services to researchers from University of Helsinki, Biocenter Finland affiliated universities and other researchers. We provide transgene ...