Change the medium 1 h before transfection. 2| To prepare the calcium phosphate–DNA coprecipitate, combine 100 μl of 2.5 M CaCl 2 with 25 μg of plasmid DNA in a sterile 5-ml plastic tube and ...

But calcium shows up in lots of other stuff too. For example, the hard part of your bones and teeth is made from calcium connected to a chemical called “phosphate”. Together, they make the substance, ...

"The second part of the problem is that phosphate is usually low in the environment, and the only real exception we know ... by itself because it binds to calcium to form calcium phosphate ...