BSA for non-specific binding controls: one or two different sources of BSA are spotted at 0.5 mg/ml as controls. • RPPA cell lysis buffer as a negative control. Total protein staining is done with ...

Otherwise, continue this procedure during the afternoon lab period and then begin the plasmid isolation protocol. 4. After the incubation period, cool the solution to room temperature. Add 20 µl of ...

Scrape each plate of cells into 1 ml of ice-cold EBC lysis buffer (50 mM Tris (pH 8 ... sequencing on an ABI 477A or 494A machine. This protocol was adapted from “Identification of associated ...