“In the war in the Crimea, the British used chloroform almost universally in their operations; the French also exhibited it very extensively, and Baudens, one of their leading military surgical ...

“In the war in the Crimea, the British used chloroform almost universally in their operations; the French also exhibited it very extensively, and Baudens, one of their leading military surgical ...

Cells are homogenized in guanidinium thiocyanate and the RNA is purified from the lysate by extraction with phenol-chloroform at reduced pH 1. This method allows many samples to be processed ...

The method of choice is a one phase extraction, as pioneered by Folch and modified by Bligh and Dyer (1959). Water, methanol, and chloroform are mixed in the ratio 1:2:0.8. Under these conditions, all ...