In confocal microscopy, the optical-sectioning ability is created by placement of a field stop, the so-called pinhole, in a conjugate image plane in front of a detector along the fluorescence beam ...

Using lasers for fluorescence applications requires special consideration of the optical path. This special attention ... some flow systems and newer confocal microscopes use mirrors at 10 ...

While confocal microscopy uses a pinhole to reject out-of-focus light to generate the optical section, a multi-photon (or 2-photon) microscope uses a pulsed infrared laser to stimulate fluorescence ...

The key advantage of the confocal principle is the ability to collect high-resolution, serial optical sections from thick specimens. That stands in stark contrast to traditional microscopic techniques ...

Our research interests include extending the depth of field of imaging systems, increasing image resolution beyond Rayleigh's resolution criterion, and quantifying the optical path length ...

As [Zachary Tong] points out, confocal scanning microscopy uses a clever optical setup to collect light from a single, well-defined point within a sample; rather than getting an image of all the ...

The Leica SP8 is a dual purpose visible confocal microscope and multi-photon on a single platform. The confocal microscope is equipped with 4 detectors, one of which is a highly sensitive hybrid ...

with motorized optical zoom, the system delivers industry-leading signal to noise and image fidelity. Applications ranging from single molecule to live cell confocal, TIRFM to whole embryo and ...