You have full access to this article via your institution. So the question remains "What are the essential prerequisites for establishing a quantitative RT-PCR assay?" To date, a great deal of ...

Real-time PCR combines the amplification of a DNA sequence with the detection of the amplified products during each reaction cycle—in other words, in real time. In comparison to conventional PCR ...

This technique allows scientists to see the results almost immediately while the process is still ongoing, whereas conventional RT–PCR only provides results at the end of the process. Real time RT–PCR ...

Although the SYBR Green I-based real-time PCR assay is less specific than the TaqMan real-time RT-PCR, it does have certain advantages, such as reduced expense (especially for high throughput ...

Multiplex real-time PCR-based diagnostic tests facilitate the simultaneous detection of multiple pathogens in a single assay, ...

or real-time RT–PCR. Fast and accurate, real-time RT–PCR has been critical in detecting, tracking and studying COVID-19. But what is it, and how does it work? Let us explain.

Note: Sequences in parenthesis represent probe sequence used in real-time PCR. * This RT-PCR assay was performed with the primer sets as described but in *, another round of hemi-nested PCR ...

The Asia-Pacific region will witness the highest growth. The PCR technologies market is expected to grow to $21.89b by 2030, at a compound annual growth rate of 7%, according to Markets and Markets.