Throughout the PCR process, DNA is subjected to repeated heating and cooling cycles during which important chemical reactions occur. During these thermal cycles, DNA primers bind to the target DNA ...

The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...

If both the emulsified and nonemulsified reactions have failed then the PCR strategy may be flawed: consult the instructions provided by the manufacturer of the DNA polymerase.

PCR involves the enzymatic amplification of nucleic acid templates, and can be divided into four major steps, listed below. 1 Denaturation: Double-stranded DNA separates into single strands. Annealing ...

PCR amplifies DNA in a three-step process: denaturation, which melts double-stranded DNA into single strands; annealing, which lets small pieces of primer DNA bind to either side of the region of ...

At the Nevada Genomics Center we offer DNA sequencing using dye-terminator Sanger sequencing with analysis on an Applied Biosystems SeqStudio Genetic Analyzer. A Sanger sequencing reaction is run with ...

PCR-machines are used for replication of DNA, detecting DNA sequences, carrying out DNA fingerprinting, forensic analysis, and molecular cloning, diagnosing genetic diseases, and detecting ...