If both the emulsified and nonemulsified reactions have failed then the PCR strategy may be flawed: consult the instructions provided by the manufacturer of the DNA polymerase.

such as DNA and RNA. PCR involves the enzymatic amplification of nucleic acid templates, and can be divided into four major steps, listed below. 1 Denaturation: Double-stranded DNA separates into ...

DNA sequences were first amplified by high–fidelity PCR using Pfu polymerase; they were then used both as 'megaprimers' and templates in subsequent asymmetric long PCR amplifications to form ...

A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...

The precise insertion of DNA sequences using the homology ... HEK293 cells using electroporation with 5 ug of PCR dsDNA and GenWand™ dsDNA templates. Image Credit: GenScript ...