Washes are carried out to remove contaminants and the DNA is eluted through the column in a high pH buffer. 1. In a sterile 1.5 ml Eppendorf tube, add 180 µl of Lysis Solution T (for tissue) and 20 µl ...

最近，Eppendorf公司推出了一种新型质粒DNA抽提系统-FastPlasmid试剂盒 ... 6、将反应后（裂解）的菌液转移到装在Wash Tube中的Spin Column中。 7、高速离心30-60s。 8、加入400 ml DILUTED Wash Buffer。 9、高速离心30-60s。 10、取出Spin Column，轻轻倒出洗液，再将Spin Column放回 ...