There are many popular chemistries for real-time PCR. One class uses different fluorescent dyes incorporated in short oligonucleotide probes specific for the amplified target. The second class ...

The assay sensitivity was 20% using probes complementary to the wild-type ... A.A. & Rasmussen, R.P. Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques 22, 130 ...

How it Works: Real Time PCR Brendan Maher The instrumentation is basic: a thermal cycler for amplification, a light source for excitation of fluorescent probes (see chemistries below), a camera for ...

qPCR, also known as real-time PCR, is an extension of traditional PCR, where DNA amplification over time is quantitated by measuring fluorescence from dyes intercalating into nascent DNA strands or by ...

Molecular Beacons do not rely on probe hydrolysis from the 5' exonuclease activity of Taq polymerase to generate fluorescence, therefore, a post-PCR melt curve is possible. Just like standard dual ...

Full range of fluorescent dyes to ensure optimal compatibility ... Thanks to the large array of dyes they offer, you can develop the ideal PCR or qPCR probe for your application without losing ...