The assay sensitivity was 20% using probes complementary to the wild-type ... A.A. & Rasmussen, R.P. Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques 22, 130 ...

There are many popular chemistries for real-time PCR. One class uses different fluorescent dyes incorporated in short oligonucleotide probes specific for the amplified target. The second class ...

Initially, ethidium bromide (EtBr) was commonly used as a DNA-binding fluorescent dye for monitoring qPCR ... the sensitivity and precision of digital PCR. Digital PCR techniques, like ddPCR, also ...

qPCR, also known as real-time PCR, is an extension of traditional PCR, where DNA amplification over time is quantitated by measuring fluorescence from dyes intercalating into nascent DNA strands or by ...

Full range of fluorescent dyes to ensure optimal compatibility ... Thanks to the large array of dyes they offer, you can develop the ideal PCR or qPCR probe for your application without losing ...

The fluorescence in situ hybridization (FISH) technique works by detecting fluorescent signals generated when probe molecules bind ... polymerase chain reaction (PCR) analysis, and it holds ...