There are many popular chemistries for real-time PCR. One class uses different fluorescent dyes incorporated in short oligonucleotide probes specific for the amplified target. The second class ...

PCR genetic analysis has been in the spotlight since COVID-19, but light is now further facilitating PCR-free methods. Osaka ...

The assay sensitivity was 20% using probes complementary to the wild-type ... A.A. & Rasmussen, R.P. Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques 22, 130 ...

is an extension of traditional PCR, where DNA amplification over time is quantitated by measuring fluorescence from dyes intercalating into nascent DNA strands or by cleavage of target-specific probes ...

Initially, ethidium bromide (EtBr) was commonly used as a DNA-binding fluorescent dye ... Digital PCR techniques, like ddPCR, also offer the ability for absolute quantification of molecules. TaqMan® ...

Light accelerates DNA hybridization using gold nanoparticles and polystyrene microparticles as probes for PCR-free DNA detection.

The fluorescence in situ hybridization (FISH) technique works by detecting fluorescent signals generated when probe molecules bind ... polymerase chain reaction (PCR) analysis, and it holds ...

Full range of fluorescent dyes to ensure optimal compatibility ... Thanks to the large array of dyes they offer, you can develop the ideal PCR or qPCR probe for your application without losing ...

When the two probe molecules bind to the target, fluorescent signals are emitted ... tools – blood tests and polymerase chain reaction (PCR) analyses can take days to weeks.