FITC, Cy3, Cy3.5, Cy5, and Cy5.5 (Chroma Technology, Brattleboro, Vermont). A 75-W Xenon lamp was used to capture all of the fluorochrome channels. The individual fluorochrome channels from the ...
This leads to the first rule of reagent selection: pick the brightest fluorochromes. For example, in a four-color panel using fluorescein ... For example, PE-Cy5 has a very bright stain index ...