Freezing medium: 90% fetal calf serum, 10% DMSO (sterilized, 0.22-μm filter) HSF-6 freezing medium: 90% fetal bovine serum (heat-inactivated; HyClone), 10% DMSO (sterilized, 0.22-μm filter ...

This method has the advantages of simplicity and safety, but does not freeze the tissue as rapidly as immersion in a freezing medium. Isopentane also can be chilled in liquid nitrogen (-176C). With ...