Figure 1: Retroviral backbone of pSHAG-MAGIC2 ... in length from the coding sequence of the gene of interest for each clone to be constructed. Only coding sequences are targeted, and each shRNA ...

Transgenic animals continue to play an essential role in many aspects of zebrafish research, including the development of disease models. The most widely used system for zebrafish transgenesis is the ...

A technology that uses enzymes to cut and paste together DNA sequences of interest. The recombined DNA sequences can be placed into vectors that carry the DNA into a host cell. In this host cell ...