To generate the hairpin primer, select a 'sense' sequence(s) of 22 nucleotides (nt) in length from the coding sequence of the gene of interest ... miR-30 shRNA cloning experiments with reasonable ...

In principle, cloning PCR products necessitates some ... new strategies for mapping behavioral mutants to cope with genetic background issues and the variance of phenotypic assays, much more ...

Cloning is a biotechnological process that involves creating genetically identical copies of an organism or cell. In essence, a clone is an exact genetic replica of the original organism or cell, ...

Gene Cloning creates copies of genes or DNA fragments. Gene cloning is the most common form of cloning performed by researchers at the National Human Genome Research Institute. Genes are cloned ...

Gibson Assembly Cloning is an elegant and robust seamless cloning methodology that has been widely adopted by the scientific community and enables the assembly of multiple DNA fragments of varying ...

Topics will include gene cloning, PCR, recombination, transposons, transgenes and mutagenesis using state-of-the-art technologies like CRISPR and gene drives. Students will gain practical experience ...