The beginning of One Piece‘s new chapter checked in on Kiku after her arm was amputated. She is clearly in shock following the attack, and her older brother is the first to give her first aid.

This is a very strong indication that Oda respects their gender identities, since Kiku is undeniably a trans character. One Piece is a Japanese series written by Eiichiro Oda. Of course ...

Many One Piece characters ... while her brother Izou was unceremoniously killed off by a few smaller attacks. During the climactic Raid on Onigashima, Kiku received two powerful and deadly attacks ...