To prepare the cloning vectors ... pMRBAD constructs with 35 μg/ml kanamycin. Prepare plasmid DNA using standard miniprep protocols. pMRBAD-link-CGFP transformations must be incubated with ...

Figure 1: Retroviral backbone of pSHAG-MAGIC2. To generate the hairpin primer, select a 'sense' sequence(s) of 22 nucleotides (nt) in length from the coding sequence of the gene of interest for ...