1| Prepare a 40-ml 4.5% native acrylamide gel (using 1 to 1.5-mm spacers): Prerun the 'gel for 2 h at 10 mA. Large protein complexes greater than 1 MDa in mass are difficult to resolve and migrate ...

The first step was to obtain the protein in its native form. To do this ... ion exchange chromatography, and gel filtration. Each step of the procedure was aimed at obtaining a stable and functional ...

Sample preparation, including protein extraction and quantification Protein separation via gel electrophoresis Membrane transfer of proteins from the gel Immunoblotting with primary and secondary ...

His-tagged EYFP–DEVD–EGFP in the absence or presence of recombinant caspase-3 (50 ng/ml) was resolved on a 12% native gel at pH 8.8, and the relevant fluorescent protein bands were excised and ...

FermWhey Native offers a high-purity beta-lactoglobulin ... acids and reduce dairy’s nutritional value. Finally, FermWhey Gel is designed to offer advanced gelling properties, allowing ...