We have developed a two-step, real-time, quantitative assay, using the polymerase chain reaction with reverse transcription (RT-PCR) and based on SYBR Green I monitoring of PCR product ...

Real-time PCR is the favored method for measuring gene expression. Researchers benefit from a vast and growing choice of reagents and instruments for their experiments. Laura Bonetta reports.

led to the discovery that PCR amplification can be monitored in real time using fluorogenic nucleic acid probes ... Its drawbacks, including being a potential human mutagen, led to the introduction of ...