Real-time PCR combines the amplification of a DNA sequence with the detection of the amplified products during each reaction cycle—in other words, in real time. In comparison to conventional PCR ...

We have developed a two-step, real-time, quantitative assay, using the polymerase chain reaction with reverse transcription (RT-PCR) and based on SYBR Green I monitoring of PCR product ...

为解决鸡星状病毒(CAstV)在厄瓜多尔家禽业中的诊断空白问题，厄瓜多尔拉斯美洲大学团队开发了基于SYBR? Green的快速RT-qPCR检测技术。研究对120例7日龄腹泻雏鸡空肠样本进行检测，发现70.83%样本携带高浓度CAstV(最高达9.6×10 7 拷贝数)，首次证实CAstV在厄瓜多尔的 ...

The fluorescent chemistry coupled with advanced optical detectors renders such techniques more sensitive than conventional gel-based PCR. Over the past 2 years, several real-time PCR assays have ...

In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.

The development of the polymerase chain reaction (PCR) in 1983 by Kary Mullis and coworkers revolutionized molecular biology, allowing scientists to amplify DNA sequences for various applications. 1 ...