Place the tube with the sheared DNA (Step 17) in the thermal cycler and carry out cycle 1 of the program through the first minute at 10 °C. Press 'Pause' to interrupt the program, and then ...

This procedure includes DNA fragmentation (sonication, nebulization or shearing) followed by DNA repair and end-polishing (blunt-end or A-overhang) and finally platform-specific adaptor ligation.