Resuspend the cells very gently and centrifuge them for 5 min at 200g. Remove the supernatant and resuspend in 2 ml freezing medium per well. The size of the cell colonies on replating is critical ...

Its product offerings include proprietary hypothermic storage and cryopreservation freeze media products for cells, tissues, and organs, generic blood stem cell freezing and cell thawing media ...

As the temperature drops, extracellular water begins to freeze, leaving behind a slush of concentrated solutes. In an attempt to dilute those solutes, water rushes out of the cell (3), causing ...