All stainings are carried out at 4°C. Sometimes, higher intensity tetramer stains are obtained if incubated at room temperature or above, but some surface markers — particularly CD62L— are sensitive ...

The cost of making a Phycoerythrin conjugated tetramer is $800. The most expensive reagents are the biotin ligase (BirA from Avidity, LLC) and the PE-conjugated streptavidin (Prozyme). On occasion, it ...

However, most TCRs produced previously have affinities that are too low for target detection under normal assay conditions, and limited stability (due to their generation ... such as cancer- or ...