The cost of making a Phycoerythrin conjugated tetramer is $800. The most expensive reagents are the biotin ligase (BirA from Avidity, LLC) and the PE-conjugated streptavidin (Prozyme). On occasion, it ...
We will perform the subcloning to the appropriate vector for protein expression and additional charges may apply. All expression plasmids containing MHC alleles will be retained at our facility and ...
All stainings are carried out at 4°C. Sometimes, higher intensity tetramer stains are obtained if incubated at room temperature or above, but some surface markers — particularly CD62L— are sensitive ...
“It's the best ligand-protein binding pair in all of biology ... which interact very tightly to form the tetramer. Previous attempts to stabilize the dimer have involved engineering strategies ...
Technically, Pol-theta is an enzyme—a type of protein that speeds up chemical ... has shown that Pol-theta exists in two ...
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