SINCE the introduction of thin sectioning by conventional methods for electron microscopy in 1948 1, several embedding techniques have been described 1,2. In one of these, namely, double embedding ...

Routine preparation of biological material (tissues, suspensions and cell monolayers grown on glass coverslips) for classical transmission electron microscopy (TEM) involves tissue fixation, embedding ...

Use a sharp blade such as this one, which has a handle with replaceable blades. Handles and blades are available from vendors of histology and pathology supplies. If there is an area of particular ...

There are many variables affecting the quality of frozen sections. You may have to experiment with different methods. Be advised that it can be difficult to get acceptable sections from some tissues.