In one of these, namely, double embedding in nitrocellulose and paraffin, the tissue is first infiltrated with an ether–alcohol solution of nitrocellulose ; the volume occupied by the solvent is ...

However, they can be used to maintain orientation or positioning of trimmed samples, as distortion is of less concern with fixed solid tissues. (See the examples on the Special processing & embedding ...

If the tissues were frozen in a flammable freezing medium, take care to allow it to evaporate before placing the blocks in the freezer. Even if frozen in embedding medium, tissues must be protected ...

involves tissue fixation, embedding and sectioning. Cells are typically fixed with 2% glutaraldehyde (e.g. in 100 mM Na-cacodylate or Na-phosphate buffer, pH 7.4) for 1-2 hours at RT post-fixed with 1 ...