Place the reaction in a thermal cycler, and amplify the DNA fragments according to the following touchdown program: It is possible to reduce the number of PCR cycles to minimize polymerase-induced ...
the last eight PCR cycles should be run with an annealing temperature of 53°C (the same result can be achieved with a touchdown protocol); (4) the final elongation step should not be shorter than ...
This PCR machine can run even the most complex thermal cycling techniques including time and temperature increments, touchdown PCR, and temperature gradients.
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