Serial dilutions were performed using DNA extracted from Nras mutation-bearing cell lines into placental DNA (wild-type ... mutations using our multicolor multiplex assay. Two of the cell ...

Real-time PCR detects and quantifies DNA as the reaction progresses. This quantifies the specific amount of DNA present in a sample. The procedure requires specialized thermal cyclers with ...

A major advantage of drop-off digital PCR is the single-assay detection of ... hotspot but is uniquely complementary to the wild-type sequence, and a reference probe that hybridizes adjacent ...