Although 5'-3' nuclease oligoprobe (TaqMan assay) is the most widely used format owing to its commercial utility and higher specificity as a result of sequence-specific hybridization of the probe ...

Recombinant baculovirus stocks from the transfection or co-transfection are plaque purified by an agarose overlay assay. Individual recombinant viral isolates are plucked from agarose and used to ...