Table 1: Common qPCR detection methods with their pros and cons Hairpin-shaped probes with a fluorophore at one end and a quencher at the other end Upon binding to the target, the loop straightens, ...

Such probes consist of a 5' fluorophore dye, a 3' Black Hole Quencher (BHQ), and an internal nova quencher integrated between base residues 9 and 10. Image credit: LGC Biosearch Technologies When ...

When coupled with a non-fluorescent quencher like EDQ ... MGB ValuMix assays for qPCR gene expression offer the convenience of receiving a customized, dual-labeled MGB probe and primer pair ...

Until this past January, few outside academic and diagnostic labs knew what quantitative reverse-transcription PCR (RT-qPCR) was, much less its role in viral and bacterial disease detection.