Until this past January, few outside academic and diagnostic labs knew what quantitative reverse-transcription PCR (RT-qPCR) was, much less its role in viral and bacterial disease detection.

She is passionate about making complex scientific concepts accessible to readers. View Full Profile. Learn about our Editorial Policies. Quantitative polymerase chain reaction (qPCR) is a molecular ...

1 Roughly a decade later, research by Higuchi et al. led to the discovery that PCR amplification can be monitored in real time using fluorogenic nucleic acid probes, allowing for quantitative ...

The PCR cycling reactions are carried out in an automated thermocycler, which is capable of heating and cooling the reaction tubes in a short span of time. PCR-Machines are classified depending on ...