In contrast, off-target effects for siRNA are much more difficult to classify, as short stretches of sequence homology may exist among RNA molecules encoding structurally distinct classes of ...

(Table 1) The efficiency and specificity of RNAi depend on the position and sequence of the siRNA used. So how do you pick out the right 21-nucleotide sequence to knock down your favorite gene?

They observed that introducing double-stranded RNA (dsRNA) into the worm led to the silencing of genes with complementary sequences. This discovery laid the foundation for understanding the mechanism ...

These siRNAs are then incorporated into siRNA – ribonuclease complexes (RISC). If the siRNA sequences significantly match host mRNAs, RISC may target them for degradation leading to disease symptoms.

In SNALPs, the backbone-modified siRNA is encapsulated within a closed shell of a cationic-zwitterionic lipid bilayer, furnished with an outer PEG shield (Figure 2A). The lipid bilayer contains a ...

By selectively modulating T cells that drive disease pathology, ctLNP-siRNA therapeutics could potently block target function with sequence-level specificity while sparing the broader immune system.