1| Gather the IEF gel tubes and set up the IEF casting apparatus. A handy casting device (described in ref. 2) fills up to 15 gel tubes automatically. The IEF tubes are placed upright in the tube ...
5 | Place the gel in the TAFE apparatus filled with 1× TAFE gel buffer, cooled to 14 °C. 6 | Connect the gel apparatus to an appropriate power supply set to deliver 4-s pulses at a constant ...
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