9| Cast the slab gels as for one-dimensional SDS-PAGE (Laemmli or tricine procedure) and assemble the gel cassettes into the second-dimension electrophoresis apparatus. Fill the electrode chambers ...

SDS, 1 μg/ml poly(A), 100 μg/ml salmon sperm DNA, 50% (vol/vol) formamide). This protocol was adapted from “Pulsed-field gel electrophoresis via transverse alternating field electrophoresis ...