In this manuscript the authors established a novel three-dimensional culture system for stratified epithelia that allows epithelial cells to undergo epithelial-to-mesenchymal transition (EMT) and ...

2′3′ cGAMP (catalog # tlrl-nacga23-02), 3′3′cGAMP (catalog # tlrl-nacga), R848 ... the cells were washed with ice-cold PBS and directly lysed in 150 µl of sample loading buffer containing 0.1 M ...

Without washing, 3.6 ml of DMEM media containing 20% FBS was added ... Five per cent β-mercaptoethanol and 0.001% bromophenol blue were then added, and samples were boiled at 95°C for 5 min. For ...