Embryos were fixed for 30 min at room temperature with 4% paraformaldehyde in MOPS buffer (0.1 M 3-(N-morpholino) propane sulfonic acid), adjusted to a pH value of 7.5, and washed in PBT two times.

Paleontologists have found three lacewing larvae with large forward-directed stemmata (eyes in holometabolans) in 100-million-year-old Kachin amber from Myanmar. These specimens demonstrate the ...