Expanding CRISPR's Potential Since its discovery, CRISPR (“Clustered Regularly Interspaced Short Palindromic Repeats”), which ...

This study designs a DNA logic gate system integrating nucleic acid aptamers, catalytic hairpin assembly (CHA), and CRISPR-Cas12a, pioneering its use for AML antigen detection. The system comprises ...

为解决 CRISPR-Cas12a 在基因编辑中脱靶切割及 R 环形成机制不明的问题，研究人员开展了 Cas12a R 环形成动力学和力学的研究。结果发现其 R 环形成有多步中间态，受多种因素影响，相关模型揭示了其活性和特异性的机制，为基因编辑提供理论基础。 在生命科学的 ...

Department of Laboratory Medicine, Center for Single-Cell Omics and Tumor Liquid Biopsy, Zhongnan Hospital of Wuhan University, Wuhan 430071, China ...

目前，还没有针对 DENV 的有效疫苗和特效治疗药物，因此早期检测就显得尤为重要。然而，传统的检测方法，如病毒分离、血清学检测、酶联免疫吸附试验、逆转录聚合酶链反应（RT-PCR）和实时定量 RT-PCR（qRT-PCR）等，存在着诸多不足。这些方法要么依赖特定的 DENV 抗体、昂贵的设备，要么需要专业技术人员操作，在资源有限的地区难以推广使用。

CRISPR-Cas systems have been exploited for targeted genome editing. CRISPR-Cas12a is a programmable endonuclease used for genetic engineering. Here, the authors use real-time single-molecule ...

Using gene editing with CRISPR-Cas9, the team has been able to study these proteins without altering ... Dec. 13, 2024 — Microorganisms produce a wide variety of natural products that can be ...

A pipeline for the analysis of CRISPR edited data. It allows the evaluation of the quality of gene editing experiments using targeted next generation sequencing (NGS) data (`targeted`) as well as the ...