Expanding CRISPR's Potential Since its discovery, CRISPR (“Clustered Regularly Interspaced Short Palindromic Repeats”), which ...

This study designs a DNA logic gate system integrating nucleic acid aptamers, catalytic hairpin assembly (CHA), and CRISPR-Cas12a, pioneering its use for AML antigen detection. The system comprises ...

为解决 CRISPR-Cas12a 在基因编辑中脱靶切割及 R 环形成机制不明的问题，研究人员开展了 Cas12a R 环形成动力学和力学的研究。结果发现其 R 环形成有多步中间态，受多种因素影响，相关模型揭示了其活性和特异性的机制，为基因编辑提供理论基础。 在生命科学的 ...

Department of Laboratory Medicine, Center for Single-Cell Omics and Tumor Liquid Biopsy, Zhongnan Hospital of Wuhan University, Wuhan 430071, China ...

目前，还没有针对 DENV 的有效疫苗和特效治疗药物，因此早期检测就显得尤为重要。然而，传统的检测方法，如病毒分离、血清学检测、酶联免疫吸附试验、逆转录聚合酶链反应（RT-PCR）和实时定量 RT-PCR（qRT-PCR）等，存在着诸多不足。这些方法要么依赖特定的 DENV 抗体、昂贵的设备，要么需要专业技术人员操作，在资源有限的地区难以推广使用。

Cas12a tool to enable simultaneous genetic modifications, allowing the study of complex genetic interactions in immune system ...

Advances in the gene-editing technology known as CRISPR-Cas9 over the past 15 years have yielded important new insights into ...

RAA preamplification combined with the CRISPR/Cas12a detection system has been used for many viruses detection, for example, SARS-Cov-2 (Xiong et al., 2020), African swine fever (Bai et al., 2019), ...