Cells navigating in complex microenvironments frequently encounter narrow spaces that physically challenge migration.

1| Incubate the cell lysate with 50 μl of a 50% slurry of ... 7| Wash the beads four times with 1 ml of ice-cold GST lysis buffer. Centrifuge the tubes at maximum speed for 1 min in a ...

One of the most picturesque spots on Capri, Villa Lysis offers a bird's-eye view of the towns of Capri and Anacapri, as well as Marina Grande. The expansive grounds of this 20th-century villa ...

Following the application of stretch, the cells were rinsed with PBS before being exposed to a lysis buffer, a combination of RIPA lysis buffer and 1% protease inhibitor (both from Fisher Scientific).

Cell death is achieved by two fundamentally different ... Beads were washed four times with the lysis buffer. Proteins were separated with SDS-PAGE and blotted onto nitrocellulose filters.

Abstract Research of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) and Fibromyalgia (FM), two acquired chronic illnesses affecting mainly females, has failed to ascertain their frequent ...

This had never happened before. There is a remarkable story behind these cells; it's more than half a century old, but still relevant and current. Dr Maninder Ahluwalia, senior lecturer in ...