FITC, Cy3, Cy3.5, Cy5, and Cy5.5 (Chroma Technology, Brattleboro, Vermont). A 75-W Xenon lamp was used to capture all of the fluorochrome channels. The individual fluorochrome channels from the ...
Hundreds of fluorochromes are available to users conducting flow cytometry assays, each with unique properties that influence panel resolution and data quality. Selecting the right combination can be ...
Imaging was performed on a custom build setup based on an automated Nikon Ti Eclipse microscope equipped with appropriate dichroic and filters (ET dapi/Fitc/cy3 or ZET405/488/561/640m dichroic, ...