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《揭秘乙肝病毒组装之谜:LHBs 蛋白如何招募衣壳开启关键进程》
研究人员为了追踪核心蛋白和 LHBs 蛋白在细胞内的路径,确定它们在哪个细胞区室相遇并促进 HBV 病毒颗粒的形成,开展了一系列实验。他们主要运用了以下几种关键技术方法:一是利用荧光共振能量转移(FRET)和荧光寿命成像显微镜(FLIM)技术,可视化两种蛋白的相互作用;二是借助透射电子显微镜(TEM)和免疫 TEM ...
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