Functional validation employed in vitro assays (proliferation, migration, invasion, drug sensitivity) and xenograft models. Mechanistic studies involved luciferase reporter assays, RNA ...

After 48 h, the medium was removed and the cells were washed and lysed. Luciferase activity was measured using a luciferase assay kit, according the manufacturer’s instructions (Promega, Madison, USA) ...

PPA2 inhibits glycolysis-mediated CRC metastasis by facilitating the degradation of HIF-1α To investigate the impact of NEDD4 phosphorylation at T758 on its ubiquitination and protein degradation ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Global hepatic DNA methylation change has been ...

Here, we investigate the mechanisms of action of Δ133p53α and Δ160p53α isoforms using chromatin immunoprecipitation, luciferase expression, subcellular fractionation, immunofluorescence assays, and ...

and luciferase activities in cell lysates were measured at 20 h post-transduction to calculate infection (%) relative to nonantibody-treated controls. The results are expressed as the mean ± SD of ...

Correspondence to Professor Alfred Sze-Lok Cheng, School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China; alfredcheng{at}cuhk.edu.hk; Professor Joseph Jao-Yiu Sung, Lee ...

The vagina is a flexible tube that joins the uterus to the vulva. Vaginas are usually around 3 inches long but may vary widely in color, size, and shape. The vaginal opening is the part of the ...

Laboratory of Medicinal Chemistry, Faculty of Pharmacy and Food Sciences and Institute of Biomedicine (IBUB), Universitat de Barcelona, Av. Joan XXIII 27-31, 08028 Barcelona, Spain ...

12 Hanyang University Institute for Rheumatology Research, Seoul, South Korea ...