在我们身体里，每个细胞都是一座蕴含无数奥秘的小宇宙。然而，这些细胞尺寸大多在微米级别，且十分透明，在显微镜下就像是一张张黑白照片，过去科学家们很难分辨其结构与细节。 直到细胞染色技术出现，才为这个微观世界带来明亮光彩，让我们得以一探究竟。

MitoTracker® Green FM是一种细胞渗透型的carbocyanine结构衍生物，包含标记线粒体的弱巯基反应性的氯甲基官能团。 本品是一种亮绿色荧光探针（（Ex=490 nm，Em=523 nm）），其在水溶液中几乎不发荧光，只有当聚集在线粒体的脂质环境才能发荧光，背景荧光几乎可忽略。

For mitochondrial staining, cells were incubated in full media at 37°C for 30 min in the presence of 100 nM Mitotracker Deep Red FM and 100 nM Mitotracker Green (both Invitrogen, UK). Cells were ...

CAR-T cells alone or co-cultured with SEM at a 1:1 ratio for the indicated time, and 200 nM TMRE (MedChemExpress, HY-D0985A) was used to detect CAR-T cells mitochondrial activity, 200 nM MitoTracker ...

HS 导致山羊精液质量下降 ：与对照组相比，热应激组山羊精子数量、密度、精子质膜和顶体完整性均显著降低，表明热应激影响山羊繁殖能力。 HS 诱导睾丸损伤和 SCs 数量减少 ：通过苏木精 - 伊红（HE）染色发现热应激破坏睾丸生精小管结构，免疫荧光染色显示热应激使睾丸中 SCs 数量显著减少，TUNEL 检测结合免疫组化表明热应激诱导 SCs 凋亡，透射电镜证实热应激破坏相邻 SCs 间紧密连接。

In an experiment using DMEM incubated for 24 h, HG intervention notably elevated PINK1 and LC3B expression. In addition, HG exposure reduced the levels of cytochrome c oxidase subunit 4 and decreased ...

The Walter and Eliza Hall Institute of Medical Research, Parkville 3052, Australia Department of Medical Biology, The University of Melbourne, Parkville 3010, Australia ...