After heat-induced epitope retrieval with citrate buffer, immunohistochemical staining was performed against OLFM4 (1:200, Invitrogen/PA5-32954, Carlsbad, CA, USA), proliferating cell nuclear antigen ...

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Shihabeddin et al utilized single-cell RNA-Seq analysis of adult P23H zebrafish animals to identify transcription factors (e2fs, Prdm1a, Sp1) expressed selectively in neural progenitors and immature ...

Immunohistochemistry was ... serum for 1 h at RT to reduce background staining, and incubated overnight at 4 °C with either mouse monoclonal PCNA (Santa Cruz), or mouse monoclonal MyHC-neonatal ...

1 Department of Critical Care Medicine, Shanghai Pudong New Area Gongli Hospital, Shanghai, China 2 Hebei Medical University, Shijiazhuang, China Background: Sepsis-induced cardiomyopathy (SIC) ...

The biopsy material was embedded into paraffin blocks in Uganda, and sections for anti-LANA IHC staining were prepared at Weill Cornell Medicine using an automated IHC histology stainer (Leica Bond ...

Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, Guangdong 510055, China Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-Sen University, ...

Biomarkers such as CD4, CD8, PD-L1, FOXP3, and CK AE1/3 are critical for understanding T-cell biology and tumor-immune interactions. Traditional immunofluorescence (IF) and immunohistochemistry (IHC) ...

The phase 2a protocol was lymphatic mapping without complete axillary dissection, provided the sentinel nodes were negative for metastasis by both HE and cytokeratin IHC staining. A parallel phase ...