Division of Multi-Organ Transplantation, Department of Surgery, University of California, San Francisco, United States; Bakar Computational Health Sciences Institute, University of California, San ...

For the mito stress test, the cells were exposed sequentially to oligomycin (0.5 μM), fluorocarbonyl-cyanide-phenylhydrazone (FCCP, 1 μM), and rotenone/antimycin A (Rot/AA, 0.5 μM). For the glycolysis ...

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Then, plates were incubated in a non-CO2 incubator at 37 °C for 1 h. After that, Seahorse XF calibrant was supplemented with the ATP synthase inhibitor, oligomycin (1.5 μM), uncoupler carbonyl cyanide ...