Real-time PCR combines the amplification of a DNA sequence with the detection of the amplified products during each reaction cycle—in other words, in real time. In comparison to conventional PCR ...

Image Credit: Norgen Biotek Corporation Figure 3. Identification of Fungal and Yeast Species in a Real-time PCR (SYBR Green). DNA was isolated from 50 mg samples (wet weight) of yeast (Pichia sp ...

为解决 SYBR? Green I 染料检测恶性疟原虫成本高的问题，研究人员开展了用 Diamond?核酸染料测定抗疟标准药物 IC 50 值并与 SYBR? Green I 对比的研究。结果显示二者 IC 50 值可比，Diamond?核酸染料可低成本检测疟原虫生长，为疟疾研究提供新选择。 恶性疟原虫（Plasmodium ...

The fluorescent chemistry coupled with advanced optical detectors renders such techniques more sensitive than conventional gel-based PCR. Over the past 2 years, several real-time PCR assays have ...

实时荧光定量 PCR (Quantitative Real-time PCR，qPCR) 可实时监测 DNA 扩增过程中的荧光信号，以实现对起始模板的定量分析。通过在 PCR 反应体系中引入荧光探针或染料，使得每个扩增循环中产生的荧光信号可以被实时检测，从而反映出扩增产物的量[1]。 简单来说 ...

使用经过充分优化的快速循环方式，7500 Fast系统可在短短30分钟内得到高质量的结果。7500 Fast系统可在保证提供和7500系统相同出色性能的前提下，在同天内运行更多的样品。 7500 Fast系统，先提供了包括经验证的快速试剂、以及超过700,000个TaqMan®Gene Expression Assays ...

Expression levels of integrin beta 1 (ITGB1), integrin beta 3 (ITGB3), and integrin alpha V (ITGAV) were measured by SYBR green Real-time PCR and compared to expression levels of RASF that were ...

drug discovery and forensic medicine with the use of Real Time PCR or quantitative PCR kits and thermal cyclers. Our Molecular Biology Division-has established an in-house Advanced Sequencing and ...

Future Virology. 2008;3(2):179-192. Although not listed as a hemorrhagic fever virus, illness caused by CHIKV can be confused with diseases such as dengue or yellow fever, based on the similarity ...

The hepatocytes were then ready for subsequent experiments. Total RNA was extracted using an RNA purification kit (B0004D, EZBioscience). Real-time PCR was conducted using SYBR Green following cDNA ...